Transfection of large plasmids in primary human myoblasts
β Scribed by Campeau, P; Chapdelaine, P; Seigneurin-Venin, S; Massie, B; Tremblay, J P
- Book ID
- 110038989
- Publisher
- Nature Publishing Group
- Year
- 2001
- Tongue
- English
- Weight
- 360 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0969-7128
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with respect to the size of RNA or DNA that can be Transfection of satellite cells from dog muscle (myoencapsidated efficiently, the fact that retroviruses inblasts) in primary culture has been optimized with refect only dividing cells (they are used mainly in ex vivo spect to the position of the ch
## Abstract The transfection of expression constructs encoding a variety of transgenes is a widely used method to study gene function in cultured cells. Especially when the efficiency of the knockβdown of target proteins via small interfering RNAs (siRNAs) is to be determined by quantitative Wester