Transfection and transformation of Agrobacterium tumefaciens

✍ Scribed by Holsters, M. ;de Waele, D. ;Depicker, A. ;Messens, E. ;van Montagu, M. ;Schell, J.

• Publisher: Springer
• Year: 1978
• Tongue: English
• Weight: 748 KB
• Volume: 163
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00267408

✦ Synopsis

The freeze thaw transfection procedure of Dityatkin et al. (1972) was adapted for the transfection and transformation of A. tumefaciens. Transfection of the strains B6S3 and B6-6 with DNA of the temperate phage PS8cc186 yielded a maximum frequency of 2 10(-7) transfectants per total recipient population. In transformation of the strain GV3100 with the P type plasmid RP4 a maximum frequency of 3.5 10(-7) transformants per total recipient population was obtained. Agrobacterium Ti-plasmids were introduced in the strain GV3100 with a maximal efficiency of 4.5 10(-8). These experiments provide further evidence that the Ti-plasmid is responsible for the oncogenic properties of A tumefaciens and for its capacity to induce "opine" synthesis in Crown-gall plant cells.