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Transcriptional state and chromatin structure of the murine entactin and laminin γ1 genes

✍ Scribed by Y. Chen; J.M. Keller

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
251 KB
Volume
82
Category
Article
ISSN
0730-2312

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✦ Synopsis

Abstract

The positions of nucleosomes in the proximal 5′regions of the coordinately regulated murine entactin/nidogen and laminin γ1 genes have been identified in four different transcriptional states—constitutively off, basal, induced, and constitutively induced. In the entactin gene a 450 base pair (bp) region of open chromatin is present between three positioned nucleosomes and the transcriptional start site in the basal, induced, and constitutively induced states. Additionally there is a 200 bp open chromatin region at ∼−2.1 kbp that is only present in the induced and constitutively induced states. In the laminin γ1 gene, a 650 bp region of nucleosome‐free chromatin is present between nucleosomes positioned at ∼ −750 and +120 in all transcriptionally active states. These results suggest that basal co‐expression of these genes requires sites present in these near upstream regions. The induction to high levels appears to involve additional sites and possibly the production of new and/or the modification of existing trans‐acting factors. J. Cell. Biochem. 82: 225–233, 2001. © 2001 Wiley‐Liss, Inc.

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