Transcriptional effects of 5-bromo-2′-deoxyuridine in post-implantation mouse embryos
✍ Scribed by Th. Palayoor
- Book ID
- 112652594
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 346 KB
- Volume
- 33
- Category
- Article
- ISSN
- 1420-682X
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## Abstract ## BACKGROUND: The exposure of mouse embryos to 5‐bromo‐2'‐deoxyuridine (BrdU), a model teratogen, generates oxidative stress, induces c‐Fos dependent activator protein–1 (AP‐1) DNA binding activity, and causes skeletal malformations (Sahambi and Hales, 2006). The goal of this study wa
## Abstract Day 9 mouse embryos were cultured in 50% fetal calf serum and 50% Waymouth's medium supplemented with various amounts of insulin. Unmodified medium contained approximately 25% of the normal adult mouse blood insulin. The addition of 0.1 to 1.0 μg insulin/ml to Waymouth's medium was most