Transcriptional analysis of immune-related genes in dendritic cells from hepatitis B surface antigen (HBsAg)-positive transgenic mice and regulation of Fc gamma receptor IIB by HBsAg-anti-HBs complex
✍ Scribed by Xin Yao; Xuan-yi Wang; Chao Zhao; Shu-hui Sun; Zhe-feng Meng; Ji-ming Zhang; Jian-qing Xu; You-hua Xie; Zheng-hong Yuan; Yu-mei Wen
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 448 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0146-6615
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✦ Synopsis
Abstract
To study the gene expression profiles in dendritic cells (DCs) from a hepatitis B surface antigen (HBsAg) positive host, transcriptional analysis of bone marrow ‐derived DCs from a lineage of HBsAg transgenic mice (#59) was compared to DCs from normal mice. Among the immune‐related genes, 12 were up‐regulated, and 14 were down‐regulated in transgenic mice relative to those of normal mice. The up‐regulated genes include genes encoding immunoglobulin, histocompatibility 2 (K region), and several complement component genes, while the down‐regulated genes include the TAP1 (transporters associated with antigen processing gene‐1), interferon induced gene (Ifi203), chemokine (C‐X‐C) ligands and leukocyte‐immunoglobulin‐like genes, Lck‐interacting transmembrane adaptor genes and histocompatibility 2 (Q region and T region). Since an immunogenic complex containing HBsAg‐anti‐HBs has been used as a therapeutic vaccine for clinical trial in chronic hepatitis B patients, DCs from #59 were incubated with immunogenic complex compared to those incubated with HBsAg alone. The immune‐related six genes up‐regulated with immunogenic complex treatment were Fcgr2b, Cxcl2, Fth1, Clec4n, Lilrb4, and Dbh, with Fcgr2b (Fc gamma receptor IIB) being the highest up‐regulated gene. Interestingly, levels of Fcgr2b were found up‐regulated in patients with chronic hepatitis B undergoing immunogenic complex immunization, which returned to baseline when immunization was discontinued. In conclusion, by transcriptional analysis, immunogenic complex induced up‐regulation of Fcgr2b expression both in dendritic cells from an HBsAg transgenic mouse model and peripheral B cells from patients with chronic hepatitis B, which indicates that Fcgr2b is one of the key molecules up‐regulated by immunogenic complex. J. Med. Virol. 83:78–87, 2011. © 2010 Wiley‐Liss, Inc.