Specialized transducing 2 phages have been isolated which carry the regions of the Escherichia coli chromosome containing the gene or gene-clusters for ribosomal proteins (r-proteins) S 1, $6-S 18-L9, S 16-L 19, and L28-L33. To investigate whether these phages also carry the genes for r-proteins $9,
Transcription of ribosomal protein genes carried on F′ plasmids ofEscherichia coli
✍ Scribed by Seals, A. Allen ;Champney, W. Scott ;Kushner, Sidney R.
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 806 KB
- Volume
- 150
- Category
- Article
- ISSN
- 0026-8925
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The adjacent genes rpoB and rpoC code for the beta and beta' subunits of RNA polymerase in Escherichia coli, and are cotranscribed in the order given. The nearest known genes to rpoB are rplL and rplA,J,K, which code for ribosomal proteins, and which are transcribed in the same direction as the poly
That portion of the Escherichia coli chromosome carried by a number of lambda transducing phages, all of which carry the gua operon, was mapped using restriction endonucleases. The DNA from one of these transducing phages was subcloned onto pBR322. We have identified two recombinant plasmids which c