Using pBR322 as a vector, three plasmids were constructed, pGP2, pGP3, and pGP7, containing respectively 5, 100, 700-950, and 1,000 base pairs derived from the immunity end of bacteriophage Mu. All three plasmids contain a functional repressor gene coding for a thermosensitive repressor. RNAs produc
Transcription of bacteriophage Mu
โ Scribed by van Meeteren, Rian ;van de Putte, Pieter
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 667 KB
- Volume
- 179
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A method is described for the isolation of thermoinducible defective Mu lysogens. Four of these defective lysogens were studied more extensively. By marker-rescue experiments it was shown that the strain harbouring the smallest defective prophage contains the immunity gene cts and the genes A and B;
To study the excision of bacteriophage Mu at the DNA sequence level, the Mu-derived phage lambda placMu3 was transposed to the transcribed but non-translated leader region of a plasmid-borne tetracycline (tet) resistance gene. Revertants (excision products) were then selected by Tet+ restoration of