✦ LIBER ✦
Transcription induction of c-Ki-ras with the tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) in normal and transformed liver cells
✍ Scribed by Sai On Chan; Susanna Siu Chun Wong; Desmond Chak Yew Yeung
- Book ID
- 104674150
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 776 KB
- Volume
- 117
- Category
- Article
- ISSN
- 0300-8177
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✦ Synopsis
Results from nuclear run-off assays show that exposure of hepatocytes and Reuber H35B hepatoma cells to the tumour promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), leads to enhanced transcription of c-Ki-ras gene. This increase in transcription in turn results in an accumulation of the functionally active c-Ki-ras message. The half life of c-Ki-ras message in both normal and transformed livers cells is not altered by TPA and is determined to be 3.5 hr. The induction of c-Ki-ras message is accompanied by an increase in the level of c-Ki-ras protein.