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Transcription in nuclei isolated from carrot protoplasts: effects of exogenous DNA

โœ Scribed by Roger E. Calza; Suzanne M. Oelke; Paul F. Lurquin


Book ID
115913227
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
494 KB
Volume
143
Category
Article
ISSN
0014-5793

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We have developed a simplified procedure for the detection of cellular RNA transcripts. Entire protoplasts, isolated nuclei or nuclei sorted according to cell cycle phases are directly dotted onto filters and hybridized. Transcripts are quantified thereupon without any RNA extraction methodology. Pr