The E. coli suA mutant (T82), which is a suppressor of polarity by virtue of its impaired transcription termination factor (rho) activity, is shown to be an efficient suppressor of lambdaN- mutants. The relief of the N requirement by this host is reflected in a substantial restoration of growth and
Transcription and membrane attachment of bacteriophage λ DNA in the absence of N function in the E. coli suA1 mutant
✍ Scribed by Bourguignon-Van Horen, M. F. ;Henry-Van der Loo, N.
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 548 KB
- Volume
- 176
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
In the polarity suppressor strain psuA 1, we observe a partial N independence of both transcription and DNA-membrane attachment for a lambda NN mutant. These results, in agreement with the genetical data reported by Dambly et al. (1976), suggest that the N product and rho factor are involved in the same process but may not interact directly.
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