Transcription activation at the Escherichia coli melAB promoter: interactions of MelR with the C-terminal domain of the RNA polymerase α subunit
✍ Scribed by David C. Grainger; Tamara A. Belyaeva; David J. Lee; Eva I. Hyde; Stephen J. W. Busby
- Book ID
- 110831905
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 442 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0950-382X
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The α subunit C-terminal domain (αCTD) of RNA polymerase (RNAP) is a key element in transcription activation in__Escherichia coli__, possessing determinants responsible for the interaction of RNAP with DNA and with transcription factors. Here, the crystal structure of__E. coli__αCTD (α subunit resid
## Abstract The biochemical characterization of the bacterial transcription cycle has been greatly facilitated by the production and characterization of targeted RNA polymerase (RNAP) mutants. Traditionally, RNAP preparations containing mutant subunits have been produced by reconstitution of denatu