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Transaminase inhibitors block glycolysis and G1 to S phase progression in chick embryo fibroblasts: Reversal by α-keto acids

✍ Scribed by John Groelke; Harold Amos

Publisher: John Wiley and Sons
Year: 1984
Tongue: English
Weight: 388 KB
Volume: 119
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041190121

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✦ Synopsis

Two transaminase inhibitors, aminooxyacetate and cycloserine, inhibited the initiation of insulin-stimulated DNA synthesis in chick embryo fibroblasts. This inhibition was overcome when pyruvate (4 mM), oxaloacetate (4 mM), or alpha-ketobutyrate (10 mM) was included in the culture medium with hormone and inhibitor. Aminooxyacetate also inhibited lactate production in insulin-treated cultures in the absence of added alpha-keto acid.

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## Abstract Temporal inhibition of protein synthesis with cycloheximide prevents subsequent insulin, but not serum‐stimulated DNA synthesis in G~1~‐arrested chick embryo fibroblasts (CEF). The inhibition is measured by the incorporation of ^3^H‐thymidine into acid insoluble material and confirmed b