## Abstract Generation of high titer lentiviral stocks and efficient virus concentration are central to maximize the utility of lentiviral technology. Here we evaluate published protocols for lentivirus production on a range of transfer vectors differing in size (7.5β13.2 kb). We present a modified
Transactivator and Structurally Optimized Inducible Lentiviral Vectors
β Scribed by HAACK, K
- Book ID
- 121425639
- Publisher
- Nature Publishing Group
- Year
- 2004
- Tongue
- English
- Weight
- 743 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1525-0016
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Gene transfer vectors based on retroviruses including oncogenic retroviruses and lentiviruses provide effective means for the delivery, integration and expression of exogenous genes in mammalian cells. Lentiviral (LV) vectors provide attractive gene delivery vehicles in the context of non-dividing c
## Abstract ## Background Lentiviral vectors allow gene transfer into nonβdividing cells. Further development of these vector systems requires stable packaging cell lines that enable adequate safety testing. ## Methods To generate a packaging cell line for vectors based on simian immunodeficienc