Trans effects of cellular K and Cl on ouabain-resistant Rb(K) influx in low K sheep red bleed cells: Further evidence for asymmetry of K-Cl cotransport

✍ Scribed by E. Delpire; P. K. Lauf

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 285 KB
• Volume: 419
• Category: Article
• ISSN: 0031-6768
• DOI: 10.1007/bf00370803

✦ Synopsis

The electroneutral K-CI cotransport in low K + (LK) sheep red blood cells is kinetically asymmetric and thermodynamically outward poised (Delpire and Lauf, 1991a). We have shown 1.1 previously by trans-inhibition kinetics that CI" binds prior to K + to the outside configuration of the carrier. In the present study, we confirm that K + and CI" bind randomly to the O 1.0 cytoplasmic aspect of the transporter because K + in the R" absence of CI-, and CI-in the absence of K +, trans-inhibit ouabain-resistant Rb+(K +) influx in these cells. In contrast to the trans-inhibition pattern observed outside, neither K + nor 0.9 CI-trans-inhibit K+(Rb +) influx in the presence of the cotransported ion, further supporting the asymmetry of the K-CI cotransporter.