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trans-2-Phenylcyclopropylamine is a substrate for and inactivator of horseradish peroxidase

✍ Scribed by Lawrence M. Sayre; Robert T. Naismith II; Michael A. Bada; Wen-Shan Li; Molly E. Klein; Mark D. Tennant

Book ID
113269873
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
667 KB
Volume
1296
Category
Article
ISSN
0167-4838

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N,NЈ-Dicyanomethyl-o-phenylenediamine was synthesized with a 90% yield by a reaction of o-phenylenediamine with chloroacetonitrile in triethylamine. Our experimental results showed that it was the effective fluorogenic substrate for horseradish peroxidase (HRP) and hemin. The K m for the HRP system