Tracking Genetically-Engineered Microorganisms (Biotechnology Intelligence Unit 2)

✍ Scribed by JK Jansson

• Publisher: R G Landes Co
• Year: 2000
• Tongue: English
• Leaves: 178
• Edition: 1st
• Category: Library

✦ Synopsis

This book is an unparalleled, very comprehensive survey of methods for monitoring of specific microorganisms in the environment, with great usefulness for those working in either the assessment of risks of released GMMs or with monitoring of released microorganisms in e.g. soil bioremediation or biocontrol. The aim is to provide a background in different techniques for monitoring of specific microorganisms in environmental samples along with examples of applications of these approaches. The examples include work done in laboratory model systems (microcosms) as well as documentation from actual field releases of genetically modified microorganisms. The regulatory aspects of risk assessment of genetically modified microorganisms targeted for release are also included. Therefore, this book is unique in dealing with different aspects of monitoring of GMMs and should be useful for regulatory agencies that require a summary of existing techniques and information as well as scientists that require a review of the "state-of the art" of modern methods for monitoring specific microorganisms in nature.

✦ Table of Contents

Cover......Page 1
VBNC (viable but nonculturable)......Page 2
Copyright......Page 3
CONTENTS......Page 4
CONTRIBUTORS......Page 8
PREFACE......Page 11
CHAPTER 1 Problems in Detecting Dormant (VBNC) Cells, and the Role of DNA Elements in This Response......Page 15
Gene transfer......Page 31
Nucleic acid......Page 43
Intrinsic markers......Page 67
Luminescence......Page 83
Reporter system......Page 101
GFP......Page 115
Sugar beet......Page 131
Rhizobia......Page 141
Rhizobium leguminosarum......Page 153
CHAPTER 11 The Field Release and Monitoring of GUS-Marked Rhizobial Strain CT0370......Page 159
CHAPTER 12 Regulatory Aspects......Page 167
N......Page 177
Biocontrol......Page 137
Bioluminescence......Page 84
Biosensors......Page 94
Regulation......Page 149
GFP mutant......Page 116
Cross-resistance......Page 35
DNA extraction......Page 44
Dormant cells......Page 14
European Commission......Page 151
Soil columns......Page 143
Field trials 141, 143,......Page 154
Specific hybridization probe......Page 68
Sinorhizobium meliloti......Page 142
Gfp......Page 13
Gfp gene......Page 118
Heavy metal sensor......Page 95
Kanamycin......Page 34
Luminometry......Page 89
LuxAB......Page 86
Thiostrepton......Page 32
V......Page 178
PCR amplification......Page 51
Root colonization......Page 110
Rothamsted......Page 0
Promoter probe transposon......Page 103
Quantitative PCR......Page 55
REP-PCR......Page 75
Resuscitation......Page 6
Rhizobium......Page 70
Risk assessment......Page 150
RNA......Page 46
RT-PCR......Page 76
Subtraction hybridization......Page 69