Trace analysis by simultaneous on-column injection/enrichment of massive sample volumes

✍ Scribed by Miller, R. J. ;Barringer, G. E.

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 197 KB
• Volume: 7
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240070312

✦ Synopsis

24 1k . . 8 t (minl

Figure 1 Separation of racemic CHPGA and racemic CHPAA. Stationary phase: p -cyclodextrin covalently bonded to silicagel, 5 pm (1 75 X 4.6 mm i.d.). Mobile phase: 35 pCt v/v acetonitrile and 65 pCt v/v potassiumphosphate buffer (c = 0.1 M; pH = 4.2). Flow rate: 1 mllmin. Detection: UV 205 nm. Temperature: room temperature (-21OC). Sample: 1.5 pg racemic CHPAA (1,2) and 3.5 pg racemic CHPGA (3,4) in 20 pI of mobile phase.

and CHPAA were separated quite well (Figure 1); racemic mandelic acid was only partly resolved. However, racemic tropic acid could not be separated at all under these conditions. Further research to the impact of several factors on these separations is in progress, especially in order to reduce tailing.

References

[I] K.