TPCK-induced apoptosis and labelling of the largest subunit of RNA polymerase II in Jurkat cells
✍ Scribed by Z. Fabian; P. O’Brien; K. Pajęcka; H. O. Fearnhead
- Publisher
- Springer
- Year
- 2009
- Tongue
- English
- Weight
- 460 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1360-8185
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Linker-insertion mutagenesis was used to isolate mutations in the Saccharomyces cerevisiae gene encoding the largest subunit of RNA polymerase II (RPO21, also called RPB1). The mutant rpo21 alleles carried on a plamid were introduced into a haploid yeast strain that conditionally expresses RPO21 fro