TPA enhancement of the recovery of methotrexate and N-phosphonacetyl L-aspartate resistant mouse 3T6 cell clones is associated with transient alterations of cell cycle progression
✍ Scribed by Arpad Szallasi; Margaret Fox; Anne R. Kinsella
- Book ID
- 102278084
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- French
- Weight
- 345 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
The effects of methotrexate (MTX) and N-phosphonacetyl L-aspartate (PALA) with and without 12-Otetradecanoylphorbol-13-acetate (TPA) have been tested on the cell-cycle traverse of mouse 3T6 and Chinese hamster V79 cell lines. MTX, whether administered with or without TPA, had very little effect on the cell cycle of the V79 Chinese hamster cell line. However, low M T X concentrations produced a significant G I accumulation in the mouse 3T6 cell line after 24 hr. and this was accentuated by TPA treatment. These findings parallel previous observations that TPA enhances the recovery of MTXand PALA-resistant mouse 3T6 cell clones but has little or no effect on drug-resistant colony recovery in the 'On leave of absence from the Department of Hygiene and Epidemiol-