Towards the biotechnological isomerization of branched sugars: d-tagatose-3-epimerase equilibrates both enantiomers of 4-C-methyl-ribulose with both enantiomers of 4-C-methyl-xylulose
✍ Scribed by Devendar Rao; Akihide Yoshihara; Pushpakiran Gullapalli; Kenji Morimoto; Goro Takata; Filipa P. da Cruz; Sarah F. Jenkinson; Mark R. Wormald; Raymond A. Dwek; George W.J. Fleet; Ken Izumori
- Book ID
- 104095213
- Publisher
- Elsevier Science
- Year
- 2008
- Tongue
- French
- Weight
- 238 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0040-4039
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✦ Synopsis
Microbial oxidation of 2-C-methyl-D-ribitol and 2-C-methyl-D-arabinitol by Gluconobacter thailandicus NBRC 3254 produces 4-C-methyl-L-ribulose and 4-C-methyl-D-ribulose, respectively. Further, 4-C-methyl-L-ribulose and 4-C-methyl-D-ribulose were equilibrated by D-tagatose-3-epimerase (DTE) with 4-C-methyl-L-xylulose and 4-C-methyl-D-xylulose, respectively. These transformations demonstrate that polyol dehydrogenase and DTE act on branched synthetic sugars. The green preparation of all of the stereoisomers of 4-C-methyl pentuloses illustrates the ability of biotechnology to generate novel branched monosaccharides.