Towards multimodal nonlinear optical tomography – experimental methodology

✍ Scribed by N. Vogler; A. Medyukhina; I. Latka; S. Kemper; M. Böhm; B. Dietzek; J. Popp

• Book ID: 102452487
• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 319 KB
• Volume: 8
• Category: Article
• ISSN: 1612-2011
• DOI: 10.1002/lapl.201110033

✦ Synopsis

All-optical microspectroscopic and tomographic tools reveal great potential for clinical dermatologic diagnostics, i.e., investigation of human skin and skin diseases. While optical-coherence tomography has been complemented by twophoton fluorescence tomography and second-harmonic generation tomography, a joint study of various nonlinear optical microspectroscopies, i.e., application of the recently developed multimodal imaging approach, to sizable human-tissue samples has not been evaluated up to now. Here, we present such multimodal approach combining different nonlinear optical contrast mechanisms for imaging, namely two-photon excited fluorescence (TPF), second-harmonic generation (SHG), and coherent anti-Stokes Raman scattering (CARS) into a joint microscopic experiment. We show the potential of imaging large skin areas and discuss the information obtained in a case study comparing normal skin and keloid tissue.