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Towards in vitro molecular diagnostics using nanostructures

✍ Scribed by Tetiana Kurkina; Kannan Balasubramanian


Publisher
Springer
Year
2011
Tongue
English
Weight
618 KB
Volume
69
Category
Article
ISSN
1420-682X

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A homogeneous fluorescence-based molecular beacon (MB) method has been developed for real-time monitoring of in vitro transcription reactions. MB probes are structured as target-specific antisense oligodeoxynucleotides containing a proximate fluorophore-quencher pair. Upon binding to its target sequ