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Towards high-resolution solid-state NMR on large uniformly15N- and [13C,15N]-labeled membrane proteins in oriented lipid bilayers

โœ Scribed by Thomas Vosegaard; Niels Chr. Nielsen


Book ID
110332728
Publisher
Springer Netherlands
Year
2002
Tongue
English
Weight
405 KB
Volume
22
Category
Article
ISSN
0925-2738

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Three-Dimensional Solid-State NMR Spectr
โœ Francesca M Marassi; Che Ma; Jennifer J Gesell; Stanley J Opella ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 188 KB

Uniformly 15 N-labeled samples of membrane proteins with helices aligned parallel to the membrane surface give two-dimensional PISEMA spectra that are highly overlapped due to limited dispersions of 1 H-15 N dipolar coupling and 15 N chemical shift frequencies. However, resolution is greatly improve