Towards development of colorimetric test-systems for phenols detection based on computationally-designed molecularly imprinted polymer membranes
✍ Scribed by Т.А. Sergeyeva; L.A. Gorbach; О.А. Slinchenko; L.A. Goncharova; O.V. Piletska; О.О. Brovko; L.M. Sergeeva; G.V. Elska
- Book ID
- 103847639
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- English
- Weight
- 772 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0928-4931
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✦ Synopsis
Colorimetric test-system for the express-control of phenols content in water was developed. The system consisted of free-standing molecularly imprinted polymer (MIP) membranes which contained synthetic binding sites capable of phenol binding. The MIP membranes were obtained by co-polymerisation of a functional monomer (e.g. itaconic acid, 2-acrylamido-2-methyl-1-propanesulfonic acid, acrylamide or methacrylic acid) with triethyleneglycoldimethacrylate and oligourethaneacrylate. Rational choice of the functional monomers was based on the results of computational modelling. The phenol-selective MIP membranes were used as a basis for the colorimetric test-system for measuring phenol in aqueous samples. Phenol molecules were selectively adsorbed by the MIP membranes and quantified using color reaction with 4-aminoantipyrine. The intensity of the membranes' staining was proportional to the phenol concentration in the analysed sample. The optimised colorimetric test-system based on MIP membranes demonstrated a low detection limit (50 nM), a wide working range -50 nM-0.5 mM and an excellent storage stability at room temperature (12 months). As compared to the traditional methods of phenols detection the developed test-system is superior because of simplicity of operation, small size and low cost.