Towards a Quantitative Understanding of Protein Hydration and Volumetric Properties

✍ Scribed by Lally Mitra; Jean-Baptiste Rouget; Bertrand Garcia-Moreno; Catherine A. Royer; Roland Winter

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 542 KB
• Volume: 9
• Category: Article
• ISSN: 1439-4235
• DOI: 10.1002/cphc.200800405

✦ Synopsis

Abstract

Herein, we probe by pressure perturbation calorimetry (PPC) the coefficient of thermal expansion, the volumetric and the hydration properties of variants of a hyperstable variant of staphylococcal nuclease (SNase), Δ+PHS. The temperature‐dependent volumetric properties of the folded and unfolded states of the wild‐type protein are calculated with previously published data. The present PPC results are used to interpret the volume diagram and expansivity at a molecular level. We conclude that the expansivity of the unfolded state is, to a first approximation, temperature independent, while that of the folded state decreases with increasing temperature. Our data suggest that at low temperature the defining contribution to ΔV comes mainly from excluded volume differences and ΔV for unfolding is negative. In contrast, at high temperatures, differential solvation due to the increased exposed surface area of the unfolded state and, in particular, its larger thermal volume linked to the increased conformational dynamics of the unfolded state ensemble takes over and __Δ__V for unfolding eventually becomes positive.