Towards a quantitative determination of retinoids complexed to cyclodextrin: the diphenyl polyene model

✍ Scribed by J.-C. Guilleux; K.N. Barnouin; D.A. Lerner

• Book ID: 102982278
• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 919 KB
• Volume: 292
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)00021-2

✦ Synopsis

Retinoids are prone to oxidation or isomerization and are therefore difficult to quantify in complex media . It is expected that their inclusion in cyclodextrins would increase their stability and maintain or improve their emission properties . It seems then that such complexed retinoids would lend themselves to a better quantification . A methodology has been developed with 1,6-diphenyl-1,3,5-hexatriene (DPH), a model molecule for retinoids, to follow the formation of the complex . The latter is insoluble in cold water and soluble in acetonitrile-water (1 :3, v/v) mixtures . The complexes were detected by fluorescence on thin-layer chromatography plates . Free DPH and the inclusion complex are so well separated that the quantification of the unbound molecule was carried out without interference by means of a charge-coupled device camera . Differences in the absorbances of the free and totally solubilized DPH are used to determine the quantity of included DPH. Preliminary kinetic studies suggest that the oxidation or isomerization of DPH is decreased in the inclusion complex .