Toward quality assurance for metaphase FISH: A multi-center experience

✍ Scribed by Dewald, Gordon; Stallard, Richard; Bader, Patricia I.; Chen, Kathy; Zenger-Hain, Julie; Harris, Catherine J.; Higgins, Rodney; Hirsch, Betsy; Hsu, Wei-Tong; Johnson, Eric; Kubic, Virginia; Kurczynski, T. W.; Malone, James M.; McCorquodale, D. James; Meilinger, Karen; Meisner, Lorraine F.; Moore, J. W.; Schwartz, Stuart; Siembieda, Steven; Storto, Patrick D.; Vance, Gail; Van Tuinen, Peter; Wiktor, Ann; Yung, Jar-Fee

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 721 KB
• Volume: 64
• Category: Article
• ISSN: 0148-7299
• DOI: 10.1002/(sici)1096-8628(19960906)64:4<539::aid-ajmg3>3.0.co;2-k

✦ Synopsis

ratory made 3 errors, 1 made 2, and 2 made 1 each. Most errors and inconsistencies seemed due to inexperience with FISH. The working time to process and analyze slides singly averaged 49.5 minutes; slides processed in batches of 4 and analyzed singly required 36.9 minutes. We conclude that proficiency testing for FISH using an extensive array of challenges is possible and that multiple centers can collaborate to test probes and to evaluate costs.