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Touchdown digital polymerase chain reaction for quantification of highly conserved sequences in the HIV-1 genome

✍ Scribed by De Spiegelaere, Ward; Malatinkova, Eva; Kiselinova, Maja; Bonczkowski, Pawel; Verhofstede, Chris; Vogelaers, Dirk; Vandekerckhove, Linos


Book ID
123489281
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
774 KB
Volume
439
Category
Article
ISSN
0003-2697

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Three oligonucleotide primer combinations selected from the 5' noncoding, the nucleocapsid and the putative nonstructural regions of the hepatitis C virus genome were compared in a nested polymerase chain reaction assay with respect to sensitivity and specificity for the detection of viral RNA in ch