Totally synthetic crystalline ribonuclease A

✍ Scribed by Haruaki Yajima; Nobutaka Fujii

• Book ID: 101718530
• Publisher: Wiley (John Wiley & Sons)
• Year: 1981
• Tongue: English
• Weight: 434 KB
• Volume: 20
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1981.360200910

✦ Synopsis

Abstract

Improved chemical synthesis of bovine pancreatic ribonuclease (RNase) A was achieved by applying a new deprotecting procedure with trifluoromethanesulfonic acid–thioanisole in combination with a modified air‐oxidation procedure with glutathione for the disulfide formation. After purifications by affinity chromatography, followed by ion‐exchange chromatography, a protein with the full enzymatic activity was obtained and subsequently crystallized from aqueous ethanol according to Kunitz. A totally synthetic enzyme with full RNase A activity was thus obtained in a crystalline form for the first time.