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Total synthesis by modern chemical ligation methods and high resolution (1.1 Å) X-ray structure of ribonuclease A

✍ Scribed by David J. Boerema; Valentina A. Tereshko; Stephen B. H. Kent

Publisher
Wiley (John Wiley & Sons)
Year
2007
Tongue
English
Weight
297 KB
Volume
90
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

The total chemical synthesis of RNase A using modern chemical ligation methods is described, illustrating the significant advances that have been made in chemical protein synthesis since Gutte and Merrifield's pioneering preparation of RNase A in 1969. The identity of the synthetic product was confirmed through rigorous characterization, including the determination of the X‐ray crystal structure to 1.1 Angstrom resolution. © 2007 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 90:278–286, 2008.

This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at [email protected]

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