Total internal reflection fluorescence: Measurement of spatial and orientational distributions of fluorophores near planar dielectric interfaces

Spatially resolved detection of antibody

Spatially resolved detection of antibody—antigen reaction on solid/liquid interface using total internal reflection excited antigen fluorescence and charge-coupled device detection

✍ V. Hlady; J.N. Lin; J.D. Andrade 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 729 KB

Spatially-resolved detection of antibody-antigen reactions at the solid/liquid interface was investigated by total internal reflection excited fluorescence from large area flat surfaces. Anti-HSA immunoglobulin G (IgG) antibody was immobilized at four spatially distinct spots. Binding of fluorescein