Total content of Glycerol and Diglycerol in Mono-/diglycerides after Saponification
The "Joint Committee for the Analysis of Fats, Oils, Fatty Products, Related Products and Raw Materials (GAFett)" has developed the following method for the determination of the total content of Glycerol and Diglycerol in fats and fat derivatives. It is intended to include this method in Section C, Chapter III of the German Standard Methods*. Determination by Gaschromatography of the Total Content of Glycerol and Diglycerol in Mono-/diglycerides after Saponification [C-III 20(05)].
Scope and field of application
The method is designed to determine the content of total glycerol and diglycerol in mono-/diglycerides and fat derivatives (note 9.1).
Definition
Total glycerol content is to be understood as the sum of the monoglycerol and diglycerol parts in the sample, as determined by this method. It is stated as the sum of the glycerol and diglycerol liberated after saponification/ transesterification in gram per 100 g of sample (note 9.2).
Principle of the method
The sample is saponified/transesterified with methanolic KOH. The glycerol and diglycerol content is determined by gas chromatography using n-tetradecan as the Internal Standard.
Reagents
Warning: Attention is drawn to the regulations which specify the handling of dangerous matter. Technical, organizational and personal safety measures shall be followed.
Unless otherwise stated:
-Use only chemicals of analytical grade; -Use only distilled water or water of equivalent purity; -Generally the expression "solution" means an aqueous solution.