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Topological Measurement of Protein-induced DNA Bend Angles

โœ Scribed by Leonard C. Lutter; Herbert R. Halvorson; Christopher R. Calladine

Book ID
115627513
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
693 KB
Volume
261
Category
Article
ISSN
0022-2836

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โœ Sandra S. Zinkel; Donald M. Crothers ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 881 KB

We describe a method to detennine the magnitude of protein-induced DNA bends relative to a set of standard A tract bends using comparative gel electrophoresis. The DNA bend of interest was that induced by the catabolite activator protein (CAP), the transcriptional activator protein of the lac operon