Topochemical Polymerization of 7,7,8,8-Tetrakis(methoxycarbonyl)quinodimethane

✍ Scribed by Takahito Itoh; Shinji Nomura; Takahiro Uno; Masataka Kubo; Kazuki Sada; Mikiji Miyata

• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 130 KB
• Volume: 114
• Category: Article
• ISSN: 0044-8249
• DOI: 10.1002/1521-3757(20021115)114:22<4482::aid-ange4482>3.0.co;2-d

✦ Synopsis

Delipidated HSA (~99 %) and HGG (99 %) were obtained from Sigma. Aqueous HSA and HGG solutions (0.1 mg mL À1 ) were adsorbed to the DPPC/DLPC films at 23 8C by placing a sufficiently large drop of the protein solution to cover a surface of approximately 1 cm 2 . After 15 min, the drop was blown off of the surface by using N 2(g) .

Au nanoparticles were prepared by the citrate reduction of HAuCl 4 (Aldrich). A drop of the 10 nm Au-colloid solution was deposited on the protein-covered monolayer surface. After 5 min, the drop was blown off of the substrate surface. The surface was rinsed with a drop of H 2 O and blown dry.

AFM images were acquired in air (relative humidity ¼ 25±30 %, T ¼ 21± 24 8C) by using the tapping mode of a Nanoscope IIIa-MultiMode AFM (Digital Instruments) and etched Si cantilevers (resonance frequency ¼ 300 kHz, spring constant ~42 N m À1 ). A cantilever-oscillation amplitude of 60±65 mV and an oscillation damping of 15±25 % were used.