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Tn5-rpsL: a new derivative of transposon Tn5 useful in plasmid curing

✍ Scribed by Stojiljković, Igor; Trgovčević, Z̆eljko; Salaj-S̆mic, Erika

Book ID
122541747
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
418 KB
Volume
99
Category
Article
ISSN
0378-1119

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✍ De Vos, Guido F. ;Walker, Graham C. ;Signer, Ethan R. 📂 Article 📅 1986 🏛 Springer 🌐 English ⚖ 852 KB

Two derivatives of the prokaryotic transposon Tn5 were constructed in vitro. In Tn5-233, the central area of Tn5, which carries resistance to kanamycin/neomycin, bleomycin and streptomycin, is replaced by a fragment carrying resistance to the aminocyclitol antibiotics gentamycin/kanamycin and strept