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Tn10 Transposition via a DNA Hairpin Intermediate

โœ Scribed by Angela K Kennedy; Anjan Guhathakurta; Nancy Kleckner; David B Haniford


Book ID
117268529
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
338 KB
Volume
95
Category
Article
ISSN
0092-8674

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Characterization of Tn10d-Cam: A transpo
โœ Elliott, Thomas ;Roth, John R. ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Springer ๐ŸŒ English โš– 844 KB

We have constructed a small, transposition-defective derivative of the transposon Tn10 that carries the chloramphenicol acetyltransferase gene of pACYC184. This new genetic element, Tn10d-Cam, transposes when Tn10 transposase is provided from a multi-copy plasmid. Transposon insertion mutagenesis of