TLC Separation of Glucopyranosyl Sinapate and Other Phenolic Compounds from Rapeseed

✍ Scribed by Amarowicz, R. ;Karamać, M. ;Rudnicka, B. ;Ciska, E.

• Publisher: John Wiley and Sons
• Year: 1995
• Weight: 288 KB
• Volume: 97
• Category: Article
• ISSN: 0931-5985
• DOI: 10.1002/lipi.19950970905

✦ Synopsis

a m a c ' , B. R u d n i c k a a n d E . C i s k a *

Glucopyranosyl sinapate was obtained from rapeseeds using column chromatography on Sephadex LH-20 and RP-18 semipreparative HPLC. TLC analysis of this compound, sinapic acid and phenolic compounds fractions from column chromatography were conducted on plates coated with silica gel (standard and HPTLC), with cellulose and on RP-TLC plates. Eleven various solvent systems were applied to develop chromatograms. HPTL plates were found more efficient than those coated with standard silica gel. Compared to the latter cellulose ensured worse results of TLC analysis. The best developing system for plates coated with silica gel was benzene-methanol-acetic acid (90:16:8). Separation of the compounds analysed using RP-TLC depended on octadodecylsilanization degree of silica gel on TLC plate.