TLC Characterization of Small Unilamellar Liposomes Containing D-myo-Inositol Derivatives

✍ Scribed by Eugen Brailoiu; Corina Beschea; Cristina Brailoiu; Angela Costuleanu; Catalin M. Filipeanu; Marcel Costuleanu; Bo Fallgrens; Dimitrie D. Branisteanu

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 394 KB
• Volume: 10
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(199609)10:5<233::aid-bmc594>3.0.co;2-5

✦ Synopsis

The thin-layer chromatographic (TLC) behaviour of sma!: unilamellar liposomes containing inositol phosphates (IPS) was studied. The vesicles contained different conwntrations of D-myo-inositol 1,4,5-triphosphate (IPS), D myo-inositol 1,2,6-triphosphate (a-trinositol, PP 56, a novel Perstorp Pharma derivative), wmyo-inositol 1,3,4$-tetraphosphate (IP4), D-myo-inositol 1,3,4,5,6-pentakisphosphate (IP,) and mmyo-inositol lfJ,4$,6-hexakisphosphate (IPJ. Migration of all liposome batches was compared to that of control liposomes (multilamellar and small unilamellar, both containing only triple-distilled water), and to that of free phosphatidylcholine (PC).

The same amount of lipid was used in all situations.

Thin-layer chromatography was performed with silica gel as adsorbent. The developing solvent was an n- buthano1:ethanol:water mixture in a 4:3:3 volume ratio.

At doses higher than lo-* M liposomes containing a-trinositol and IP, had a different migration than PC, MLV or SUV as well as all batches of liposomes. Physiological studies (using as model endothelized rat aorta rings) proved that in this situation they had no effects.