TLC Characterization of Liposomes Containing Angiotensinogen, Angiotensine I, Angiotensine II and Saralazin
✍ Scribed by Eugen Brailoiu; Mihai Todiras; Anca Margineanu; Marcel Costuleanu; Cristina Brailoiu; Catalin Filipeanu; Angela Costuleanu; Valeriu Rusu; Gheorghe Petrescu
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 121 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0269-3879
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✦ Synopsis
Recent studies have described intracellular binding sites for angiotensine II. In vascular smooth muscle it was found that intracellular injection of angiotensin II increases the Ca 2+ level.
An alternative method for intracellular delivery of drugs is represented by using liposomes. Thus, the aim of this study was to characterize liposomes filled with angiotensinogen, angiotensine I (Ang I), angiotensine II (Ang II), and saralasin by a TLC method and examine the physiological effects of these on rat vascular smooth muscle. Ang II (for all concentrations tested in the aqueous phase) and Ang I (for concentrations less than 10 Ϫ 4 M) did not affect the thin-layer chromatography migration of this type of vesicle, suggesting that dose-dependent effects on physio-pharmacological experiments could be studied. On the other hand, this type of experiment could not be performed for salarasin-or angiotensinogen-filled liposomes.
Administration of liposomes containing Ang II (10 Ϫ 6 M), Ang I (10 Ϫ 6 M), angiotensinogen (10 Ϫ 6 M) and saralasine (10 Ϫ 6 M) caused the contraction to isolated rat aorta smooth muscle, suggesting the presence of active intracellular binding sites.