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Titration of tannin via alkaline phosphatase activity

โœ Scribed by Yitzhak Ittah

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
376 KB
Volume
192
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

An enzyme assay for tannin is described. It is based on the following steps: (i) bovine serum albumin (BSA) is absorbed onto polystyrene microplates; (ii) tannin is bound to the BSA-coated plates; (iii) alkaline phosphatase is then interacted with the free tannin-binding sites. The method takes advantage of the multiple hydroxyl groups of tannin which can associate more than one ligand, e.g., proteins. A pH-dependent dynamic equilibrium sets up between bound and unbound tannin and is controlled only by its initial concentration.

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