Tissue-Specific Stem Cell Differentiation in an in vitro Airway Model

✍ Scribed by Zoë Prytherch; Claire Job; Hilary Marshall; Victor Oreffo; Martyn Foster; Kelly BéruBé

• Book ID: 102934940
• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 892 KB
• Volume: 11
• Category: Article
• ISSN: 1616-5187
• DOI: 10.1002/mabi.201100181

✦ Synopsis

Abstract

The respiratory tract is the primary site of exposure to airborne compounds, with the bronchial epithelium providing one of the first lines of defence. A growing need exists for an accurate in vitro model of the bronchial epithelium. Here, normal human bronchial epithelial (NHBE) cells cultured at an air/liquid interface create a fully differentiated, in‐vivo‐like model of the human bronchial epithelium. Developmental characterisation includes (i) __trans‐__epithelial electrical resistance, (ii) morphology and (iii) bronchial cell specific stains/markers. It is concluded that the basal/progenitor cells create a pseudo‐stratified, mucociliary NHBE model containing basal, serous, Clara, goblet and ciliated cells, reflective of the normal human bronchial epithelium (days 24–33 ALI culture).magnified image