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Tissue-specific expression of the β-glucuronidase reporter gene in transgenic strawberry (Fragaria × ananassa) plants

✍ Scribed by J. Schaart; E. Salentijn; F. Krens


Publisher
Springer
Year
2002
Tongue
English
Weight
362 KB
Volume
21
Category
Article
ISSN
0721-7714

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A transient ß-glucuronidase (GUS)-assay was performed to evaluate electroporation parameters and optimize DNA delivery conditions into strawberry protoplasts. Optimal GUS-activity was obtained when protoplasts were subjected to 400 V/cm for 20 ms. GUS-activity could be further increased by the addit