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Tissue-specific expression and developmental regulation of rat medium-chain acyl-CoA dehydrogenase

โœ Scribed by D.P. Kelly; J.I. Gordon; A.W. Strauss


Book ID
119442818
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
88 KB
Volume
19
Category
Article
ISSN
0022-2828

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Specific Assay of Medium-Chain Acyl-CoA
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A spectrophotometric method for assaying medium-chain acyl-CoA dehydrogenase is described. The assay measures at 308 nm the formation of cinnamoyl-CoA from 3-phenylpropionyl-CoA in the presence of phenazine methosulfate as electron acceptor. Apparent kinetic constants (Km, Vmax) determined with 3-ph