Tissue inhibitor of metalloproteinase-2 (TIMP-2) mRNA is constitutively expressed in bovine, human normal, and osteoarthritic articular chondrocytes

✍ Scribed by Muhammad Zafarullah; Suming Su; Johanne Martel-Pelletier; John A. DiBattista; Bernard G. Costello; William G. Stetler-Stevenson; Jean-Pierre Pelletier

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 672 KB
• Volume: 60
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/(sici)1097-4644(19960201)60:2<211::aid-jcb5>3.0.co;2-#

✦ Synopsis

Tissue inhibitors of metalloproteinases (TIMPs) inhibit the extracellular matrix (ECM) metalloproteinases (MMPs). To determine the source of TlMPs in synovial fluids of patients with osteoarthritis (OA), the ability of chondrocytes to express TIMP-2 and its regulation by agents found in inflammed joints was investigated. The constitutive TIMP-2 mRNA expression was demonstrated in chondrocytes from normal bovine, human OA and normal cartilage. The cross-hybridization of human and bovine TIMP-2 suggested its evolutionary conservation. Serum, IL-I, IL-6 and TGF-P were unable to augment considerably the basal expression of TIMP-2 mRNA. TIMP-1 RNA expression in chondrocytes from human OA cartilage was elevated compared to non-OA chondrocytes, while TIMP-2 mRNA levels were similar in both. IL-1 p, IL-6 and TGF-P did not affect TlMP-2 expression but TGF-P induced TIMP-1 mRNA in human OA chondrocytes. TIMP-2 and TIMP-1 are therefore differentially regulated in chondrocytes and the basal TIMP-2 levels may be needed for the cartilage ECM integrity.