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Tissue-dependent differences in dna methylation products of mice treated with methyl-labelled methylnitrosourea

✍ Scribed by Jaroslav V. Frei

Publisher: John Wiley and Sons
Year: 1971
Tongue: French
Weight: 488 KB
Volume: 7
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910070309

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Adult inbred Swiss mice were given a single carcinogenic dose of methyl‐labelled methylnitrosourea in the range of doses which induces thymic lymphomas, lung adenomas and other random malignant tumours. DNA was extracted from pooled bone marrow, spleens, thymuses, kidneys, livers, or lungs within 1 h of injection and was hydrolysed and chromatographed to demonstrate the proportions of three products: 7‐methylguanine, 3‐methyladenine, and 6‐methoxyguanine. Disproportionately high relative amounts of 3‐methyladenine were found in the tissues thought to have a role in the genesis of thymic lymphomas. 6‐methoxyguanine, which Loveless (1969) postulates to be the product crucial for carcinogenesis, was detected in the DNA of all tissues examined. The possible usefulness of this approach to the study of carcinogenesis was thus demonstrated.

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