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Tissue culture cytotoxicity assay for cyclophosphamide metabolites in rat body fluids

✍ Scribed by Fred A. Weaver; Anthony R. Torkelson; Walter A. Zygmunt; Henry P. Browder


Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
464 KB
Volume
67
Category
Article
ISSN
0022-3549

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✦ Synopsis


An in vitro cytotoxicity assay for cyclophosphamide metabolites in rat body fluids is described. Of the two tissue culture tumor cell lines employed, the Walker-256 rat carcinosarcoma was more sensitive to metabolite levels than the L-1210 mouse lymphocytic leukemia. The Walker-256 system detected cyclophosphamide metabolite levels two orders of magnitude lower than the commonly used 4-(p-nitrobenzyl)pyridine analytical procedure.