Time-sharing evolution and sensitivity enhancements in 2D HSQC-TOCSY and HSQMBC experiments

✍ Scribed by Pau Nolis; Miriam Pérez; Teodor Parella

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 679 KB
• Volume: 44
• Category: Article
• ISSN: 0749-1581
• DOI: 10.1002/mrc.1898

✦ Synopsis

Abstract

Modifications of time‐shared (TS) HSQC‐like experiments originally developed by Griesinger and co‐workers (Sattler M, Maurer M, Schleucher J, Griesinger C. J. Biomol. NMR 1995; 5: 97) are proposed to extract different types of information from a single NMR pulse scheme. It is shown that simultaneous acquisition of ^1^H,^13^C and ^1^H,^15^N HSQC‐TOCSY and HSQMBC experiments can afford experimental sensitivity enhancements of 20–40% with respect to the separate acquisition of individual ^13^C or ^15^N data. In addition, the incorporation of a number of independent editing elements can be easily used for different purposes, for instance, to assign unambiguously ^1^H, ^13^C, and ^15^N chemical shifts, to differentiate directly from relayed cross‐peaks, or to measure simultaneously long‐range proton–carbon and proton–nitrogen coupling constants. The suggested methodologies can be applied to many different classes of nitrogen‐containing compounds and illustrative examples are provided for the peptide cyclosporine as a demonstration of the performance of such experiments. Copyright © 2006 John Wiley & Sons, Ltd.