Time-resolved immunofluorometric assays for urinary luteinizing hormone and follicle stimulating hormone

✍ Scribed by James S. Kesner; Edwin A. Knecht; Edward F. Krieg Jr.

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 833 KB
• Volume: 285
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)85003-8

✦ Synopsis

The goal of this effort was to develop and validate non-radioisotopic immunoassays for measuring luteinixing hormone and follicle stimulating hormone in unextracted urine. Towards this goal, commercial time-resolved immunofluorometric assays (IFMAs) were modified. Validation demonstrated that the resultant assays were specific, sensitive, accurate, and precise. Urine matrix was shown not to interfere with the assay. Gonadotropin profiles generated using these assays conformed to those measured in urine and serum by other established immunoassays. These IFMAs afford the collective advantages of non-radioisotopic procedures and urine sample collection (convenience, noninvasiveness, integration of pulsatile secretion), plus the superior sensitivity and specificity of IFMAs. Applications include epidemiology and medicine.