Time of ovulation in the brushtail possum (Trichosurus vulpecula) following PMSG/LH induced ovulation
✍ Scribed by Glazier, A.M.
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 72 KB
- Volume
- 283
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
✦ Synopsis
This study aimed to determine the timing of ovulation in response to a new induced ovulation regime for the monovulatory brushtail possum (Trichosurus vulpecula). Ovarian stimulation was achieved by a single subcutaneous injection of 15 IU pregnant mare serum gonadotrophin (PMSG). This treatment resulted in promotion of a large number (9-16) of Graafian follicles >2mm diameter on the ovaries. Seventy-two hours later a single intramuscular injection of 4 mg porcine luteinizing hormone (LH) was administered to induce ovulation. Groups of possums were killed 24 hr post-LH injection and subsequent groups were killed at 6-hr periods up to 48 hr later. Ovulation occurred from 30 hr to 42 hr post-LH. The ovulatory success increased from 3% at 30 hr post-LH to 83% at 48 hr post-LH. Oocytes were recovered primarily from the oviducts at 36 hr post-LH. Thereafter oocytes were recovered increasingly from the uteri and by 48 hr post-LH, were only found there. The implications of these observations for artificial breeding in possums are discussed.