Time course of IL1 and IL6 synthesis and release in human bronchial epithelial cell cultures exposed to toluene diisocyanate
✍ Scribed by S. Mattoli; F. Colotta; G. Fincato; M. Mezzetti; A. Mantovani; F. Patalano; A. Fasoli
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 942 KB
- Volume
- 149
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
We have previously demonstrated that human bronchial epithelial cells release appreciable amounts of interleukin 1 (IL1) and interleukin 6 (IL6) when exposed to toluene diisocyanate (TDI) in vitro. TDI is an inflammatory and asthmogenic stimulus presumed to act at least in part through immunological mechanisms. The epithelial cell-derived IL1 and IL6 can promote T cell activation and proliferation in culture, 2nd if thiS also happens in vivo they may contribute to the persistence of the inflammatory response of the bronchial mucosa observed in TDI-sensitive asthmatics. In this study, we confirmed the release of biologically active IL1 p and IL6-like substances from bronchial epithelial cells exposed to isocyanates in vitro, and related the rate and the magnitude of the cytokine secretion with the pattern of l L l P and IL6 gene expression and the extent of epithelial cell injury. In the epithelial cell cultures exposed to TDI, there was a parallel, progressive increase in the expression of IL6 mRNA and in the secretion of IL6 protein between 48 hours and 6 days after exposure. By contrast, although increasing amounts of biologically active IL1 p were detected in the supernatants of TDI-exposed epithelial cells throughout the 6-day period following exposure, augmented levels of l L l p mRNA were only evident 6 days after exposure, suggesting that TDI exposure might have initially affected the enzymatic cleavage of the intracellular IL1 p precursor and the mechanisms which regulate the secretion of mature IL1 6.